The Dangers of Phase Contrast Microscopy

“Every time that the person before me left the scope in phase 3, and I forgot to check.”

This honestly happens to me far too frequently, you’d think I would learn to avoid it or something. I’m just going to say that I get so excited to see my little bacteria under the scope, that I can’t help myself and shove my eyes into the eyepieces as fast as possible. I mean, some of these guys have week-long doubling times!

The Unquiet Grave

A Medieval tale of a revenant… and the boy who loves it.

Introducing, in time for Halloween, a Medieval tale of a revenant… and the boy who loves it.

I did a cursory scan-and-crop of my 24 Hour Comic, “The Unquiet Grave,” for your reading pleasure.  This was the first time that I had used watercolours in… I don’t know, it must have been 12 years or something like that.  I had recently purchased a combo pack of coloured pencils, pastels, and watercolours.  I was planning on using the pencils, but ultimately decided against that, as it wouldn’t look very much like the Medieval illuminated manuscripts.  I also stole some of Doc’s watercolours 😀

Speaking of which, Doc’s 24 hour comic is amazing and he’s finished scanning his as well.

We participated in 24 Hour Comic Book Day at Mission: Comics & Art on October 20th 2012, and you can read more about that day’s adventures here.

Continue reading “The Unquiet Grave”

An Acetone Supernova in the Lab

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In an acetone supernova, an acetone supernova in the laaaaab…

Wondering what those splotches are where the ink started running on the first panel? Guess what caused that.

Welp, this is how my day has gone.  Pretty much all week, really.  I thought I had a pretty good grasp on thermodynamics, but using the rotavapor has made me question everything I’ve ever learned.

I understand why, when the vapor really gets going, it warms up the “cone of cold” and causes an eruption of dry ice and acetone.

I understand why the distillate spontaneously freezes every now and again.

But there seems to be no rhyme or reason to when the concentrate boils over or not.  I crank the temperature down to 4 degrees C, I dump ice in the water bath, and yet every time it boils out of control for about 30 minutes and then it calms down forever.

At first I thought, well duh, I boiled all the acetonitrile off so now it’s behaving.  But lo, I brought the concentrate down to the “stable state,” turned the machine off to take lunch for an hour, came back, started it up again, and oh boy it just started boiling all over again.  If I ever stop it or take any sort of break during this “stable state,” picking up where I left off means starting back at the beginning.

Thermodynamically, I cannot explain this.  Therefore, I have decided that there’s something wrong with the vacuum pump which means it needs to warm up for a half an hour before it pulls a stable vacuum in the flask.  I tried to test this, but then I realized I had to turn the pump off to pour my liquid into the flask and restart it… but I will find a way to test this hypothesis! Mark my words!